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Gram-positive bacteria retain the crystal violet-iodine complex and stain purple, whereas gram-negative bacteria stain pink.

You will be working with many pathogenic species of bacteria in the laboratory. Remember that bacteria are in the air as well as on the skin, the counter, and all objects and equipment that have not been sterilized. The most important tool for transferring cultures is the wire inoculating needle or loop. It can be quickly sterilized by heating it to red hot in a Bunsen burner flame.

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